partec cy flow space  (Partec)

Journal: Transfusion Medicine and Hemotherapy

Article Title: Procoagulant Activity of Red Blood Cell-Derived Microvesicles during Red Cell Storage

doi: 10.1159/000494367

Figure Lengend Snippet: Flow cytometry plots of RBC-MVs in packed RBCs. FSC and SSC indicates size and granularity, respectively. The MV size gate was set by 1.0 μm beads. a Two gating regions are region R1 and R2 that represents MVs and 1.0 μm beads, respectively. Based on the FSC and SSC, RBC-MVs are located lower than 1.0 µm beads. b A histogram of logarithmic forward scatter versus count, showing the distribution of MVs in comparison to the beads. c Region RN1 represents region R1 events that were labeled with conjugated FITC-AnnV. d Region RN2 represents region R1 events that were labeled with conjugated PE anti-Gly.A and indicates RBC-derived MVs. e Region RN1 represents region R1 events that were labeled with conjugated FITC anti-CD42b, which indicates platelet-derived MVs. Non-stained events are demonstrated by the left peak in figure 1 c-e.

Article Snippet: MV Quantification by Flow Cytometry Flow cytometric analysis was performed with cy flow space flow cytometry (Partec PAS, Karlsruhe, Germany) using Flomax Software.

Techniques: Flow Cytometry, Comparison, Labeling, Derivative Assay, Staining